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To obtain highly expressing cell lines, transfectants were separated into high-expressing cell-pools by fluorescent-activated-cell-sorting after surface staining with anti-FLAG tag antibodies.
High expressing cell clones were also analysed for their expression stability over time.
They demonstrated that overexpression of miR-96 in VAMP8-expressing cell lines caused dose dependent decrease in VAMP8 mRNA and protein upon expressing cell lines [ 123].
In contrary, IMM-PLGA-NPs showed lower IC50 against non P-gp expressing cell line.
In addition, MBP fusion proteins were found to be secreted from the expressing cell.
Fig. 8 NET-binding of [11C]Me@HAPTHI on human NET expressing cell membranes using a harvesting protocol.
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TAp63 expressing cell-lines are shown in green, ΔNp63 expressing cell-lines are shown in red.
The glioma cell lines were the two MRP-expressing cell lines, T98G and IN500, an MDR1-expressing cell line, CCF-STTG1, and the MRP1 MDR1-non-expressing cell line, IN157.
Arrows mark representative EGFP and hsf1 co-expressing cell.
Relative positivity of the ShakB(N+16 -expressing cell, or relative N+16 -expressinge ShakB(L)-expressing cell, leads torlarelativetionegativitytances and vice versa.
Presence of blue staining of a cell represented a positive lacZ-expressing cell.
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