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An episomal var promoter expressing a selection marker under drug pressure causes silencing of endogenous var genes, demonstrating its infiltration into the mutual exclusive counting mechanism (Voss et al., 2006).
This was accomplished by expressing a selection of key genes, i.e. (1) three native genes in the sleeping beauty mutase (Sbm) operon, i.e. sbm- ygfD- ygfG from E. coli, (2) the genes encoding bifunctional aldehyde/alcohol dehydrogenases (ADHs) from several microbial sources, and (3) the sucCD gene encoding succinyl-CoA synthetase from E. coli.
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Thus, for proteins that have many orthologs, a common strategy is to clone and express a selection to identify the orthologs with the best solubility properties.
The use of these less toxic, potent anti-HIV vectors expressing a drug selection marker is likely to enhance the in vivo efficacy of our stem cell gene therapy approach in treating HIV/AIDS.
Thymic positive selection ensures the differentiation and maturation of thymocytes expressing a self-restricted TCR; negative selection then removes those thymoctyes that have dangerously high reactivity to self [1].
A horizontal fragmentation can be expressed by a selection condition on the table.
Hence, this tag can be used for purifying proteins expressed in a selection of different host systems [ 10, 41].
Selective effect change in the frequency of a resistance allele in a population over time due to differential survival and reproduction relative to other genotypes, commonly expressed as a selection coefficient (s) in vitro or competitive index (CI) in vivo.
On ESPN's selection show last night, Auriemma expressed a veiled frustration with the selection committee about being placed with other elite teams.
Our destination vectors were engineered to express an array of selection markers through transgene independent promoters.
To our knowledge, this is the first transcriptomic study to show differentially expressed genes in a selection of tissues in the MRL/MpJ mouse by comparative analysis with two reference strains.
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