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The P300 wave is a late cortical neuropsychological event, the latency of which reflects the information processing speed and the amplitude of which expresses memory functions.
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Receptor expression analysis revealed significantly higher proportions of CCR6 and CXCR3 expressing memory CD4+ T cells in ACPA+ patients compared to ACPA− patients.
The authors suggest that synapses merely express memory that is "stored" at the genome level.
These T cells express memory cell surface markers and exhibit a Th1/Th2 mixed cytokine profile.
(B – G ) Quantification of the synaptic accumulation of BCRs in J558L cells expressing memory B1-8-IgG-BCR (B ), J558L cells expressing memory B1-8-IgG-BCR equipped with a mIgM cytoplasmic tail, termed GGM (C ), J558L cells expressing naive B1-8-IgM-BCR (E ) and J558L cells expressing naive B1-8-IgM-BCR equipped with a mIgG cytoplasmic tail, termed MMG (F ).
DOI: http://dx.doi.org/10.7554/eLife.06925.015 Next, we examined the dependence on mechanical forces in terms of the activation of memory IgE-BCRs in Ramos human B cells expressing memory B1-8-IgE-BCRs.
In contrast, it was apparent that the J558L cells expressing MMG behaved similarly to the case of J558L cells expressing memory B1-8-IgG-BCR in a force-independent manner.
These IgG-BCR-expressing GC B cells then undergo rounds of antigen-driven selection linked with somatic hypermutation, resulting in high-affinity IgG-BCR-expressing memory B cells and long-lived plasma cells.
We addressed this question using J558L cells expressing memory B1-8-IgG-BCR in the same experimental system that has been used for the naive B1-8-IgM-BCR-expressing J558L cells as described in detail above.
(A, B ) Statistical quantification of the synaptic accumulation of IgG-BCR and the volume of the contact area of J558L cells expressing memory B1-8-IgG-BCR encountering 12 pN, 43 pN, or 56 pN NP-TGT sensors.
Furthermore, a similar phenomenon was observed when examining the activation of B1-8 primary B cells expressing memory IgG-BCRs that were derived from an in vitro class-switch response following our published protocol (Liu et al., 2010b).
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