He did not remember expressing it definitively.
However as the long form is definitively expressed and acts as an activator of hedgehog targets in response to hedgehog signalling (see below), we can conclude that midline-derived hedgehog signalling in amphioxus involves activation of ventrally-expressed target genes in ventral cells, and may also involve repression of these genes in dorsal cells, or indeed of other genes in ventral cells.
Because the endogenous protein is not mosaically expressed, it is not possible to definitively resolve the expression to either the anterior or posterior face of the cells.
On the basis of these elements Zhu et al. express a certain skepticism in definitively ruling out the association between psoriasis, psoriatic arthritis, and NOD2/CARD15.
We conclude that c-KIT positive SEM, or at least tumors with simultaneously expressed c-KIT and PLAP can definitively be classified as CSEM.
Here we show more definitively that Foxc1 is expressed by brain pericytes and that it is essential for normal pericyte function in the developing brain.
To definitively identify the Foxc1 expressing cell type in the brain vasculature, we used markers of ECs (PECAM) and pericyte/vSMC (PDGFrβ, smooth muscle actin-α) in conjunction with Foxc1 immunostaining.
To definitively determine whether physiologically expressed FLT3-ITD impacts the establishment of myeloid versus lymphoid leukemia development, we crossed Flt3 ITD/ITD with Runx1fl/fl (Growney et al., 2005) and Mx1-Cre mice to induce Runx1 deletion in MPPs.
To definitively show that the luciferase/GFP expressing cells were functional stem cells, single visualized GFPhi cells were transplanted into cleared mammary fat pads.
These results, coupled with those of the cis-regulatory analysis reported previously (Cavalieri et al., 2008), lead us to definitively conclude that hbox12 is expressed in the prospective dorsal ectoderm of the early embryo.
Meanwhile, at least four more European Union members — none of them using the euro — have expressed reservations about the agreement, which only Britain definitively opposed at the summit meeting.
