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Especially, compound 2a not only exhibited strong anti-proliferation activities against the tumor cell lines which expressed wild type or mutant EGFRL858R/T790M, but also showed the most potent inhibitory activity toward wild type EGFR (IC50 = 5.06 nM).
Cells that expressed wild type HIV-1 Tat inhibited autophagy in LC3-eGFP+ MonoMac6 cells (Figure 5D).
This value approximates the λmax = 420 nm of the expressed wild type PrV (Fig. 4D, black line).
Importantly, transiently expressed wild type Smac56, like transiently or stably expressed Smac60, have an intact epitope (Fig. 7).
Generally, the resulting proportions were high (around 80% or more, Figure 5D) regardless of whether the rescuants expressed wild type or mutant endoA transgenes.
To further confirm this interaction between LdUba5 and LdUfm1, we expressed wild type and mutant forms of LdUfm1 and performed coimmunoprecipitation experiments (Figure 1E).
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Microarray analysis revealed that expression of the F170I mutant drastically altered gene expression profiles compared with expressed wild-type BAP1.
To determine the effect of the mutations on protein expression, we cloned and expressed wild-type and mutant GMPPB in HEK293 cells.
To evaluate PICT1 function, we used shRNA to inhibit PICT1 expression in gastric cancer cells that expressed wild-type TP53.
To test if this expression pattern is an artifact of overexpression, we also expressed wild-type aPKC under identical conditions, and observed striped expression of aPKC (Fig. 6L).
To rule out off-target effects of the USP15 guide RNA (gRNA), we ectopically expressed wild-type (WT) USP15 in USP15-depleted cells.
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