I couldn't express well that I wanted to move a table, so instead just moved it myself then looked at the barman, shrugged and said "look at this chaos!".
The results showed that 4B2A1 TCR in the Vβα orientation was expressed well under standard conditions, that is, in the presence of IPTG, but without addition of sugars.
Of more than 100 designed constructs, three produced protein products that expressed well.
Eighteen of the twenty-one enzymes that expressed well in E. coli were active in the presence of the IL 1-ethyl-3-methylimidazolium acetate ([C2mim][OAc]) concentrations of at least 10% (v/v), with several retaining activity in the presence of 40% (v/v), which is currently the highest reported tolerance to [C2mim][OAc] for any cellulase.
One of the physiotherapists expressed: "Well it's certain that the doctor who sees that me, my client, well he has to recover and that I refer him all over, well he will be more likely to refer to me because the client is important".
The obstacle to blame that Watson is describing here is not the thought that Harris might not be blameworthy (though he might not be), but rather the thought expressed well by the common thought that "There but for the grace of God go I".
We then analyzed an IGF1R variant lacking the entire L1 domain (IGF1R ΔL1) and found that it expressed well in HEK293 cells and was constitutively phosphorylated as judged by anti-phosphotyrosine Western blots, demonstrating that the L1 domain is essential for maintaining the inactive state of IGF1R.
These results suggest that rDGAT1 was expressed well but was extensively degraded in E. coli under the experimental conditions.
The deletion did not change isobutanol production (Fig. 3b), suggesting that YqhD was expressed well from plasmids.
(nurse, France) They [nurses] are very good at their job but…what's missing is making sure that the mum is still expressing well and that there's still milk coming out.
