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The differential expression of a selection of the genes identified as being differentially expressed was validated by applying real-time quantitative RT-PCR (qRT-PCR).

Representative genes (up- and downregulated) identified by the microarrays as differentially expressed were validated by real-time quantification PCR (RT-qPCR; Figure 3b).

In order to confirm the microarray data, the expression of ten randomly chosen differentially expressed genes was validated by realtime PCR.

Expression of differentially expressed genes was validated by real-time PCR.

A subset of differentially expressed proteins was validated by Western blot, including regulation in specific cellular compartments, potentially caused by protein translocation.

A subset of highly differentially expressed genes was validated by RT-qPCR.

The crucial role of RANKL expressed by osteocytes was validated by the severe osteopetrotic phenotype observed in mice lacking RANKL specifically in osteocytes.

The functionality of the IL-12 or CD gene products expressed from these vectors was validated by splenic interferon (IFN -γ productIFN -γ viability assays in cultured cells.

The reduction of a subset (n = 10) of the differentially expressed MYC-target genes was validated by qRT-PCR in FBXO28 depleted HCT116 and U2OS cells (Fig 3C and D; Supporting Information Fig S3B).

The expression of differentially expressed genes from microarray experiments was validated by qPCR using hydrolysis probe-based inventoried and custom designed PrimeTime qPCR 5′ Nuclease assays procured from Integrated DNA Technologies, Coralville, IA, USA.

The vascular identity of the cells co-expressing VEGFR2 and VEGFR3 was validated by positive immunostaining for VE-cadherin (lower panel in Figure 4A).