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In J1.1 cells, we expressed vector control, Casp8FL, Casp8p41, or Casp8p41 plus a dominant negative form of IκBα (IκBα 32/36A).
However, when we expressed vector alone, dominant negative Rac1(T17N), or dominant negative Rac3(T17N), we found a significant inhibition of migration compared to the vector control (Fig. 4c).
In GSDII KO mice, AAV2/8 expressing GAA with a liver-specific promoter avoided anti- GAA immunity when injected on its own; it also allowed for protection from the immune responses induced by the ubiquitously expressed vector, suggesting the induction of tolerance.
To determine a role for Cdc42 in the migration of highly metastatic cells, we expressed vector alone and a dominant negative myc-tagged Cdc42(T17N) construct in the highly metastatic MDA-MB-435α6HG6 cell variant and subjected both to a migration assay.
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Bar graphs display the fold change in each RNA (y axis) normalized to the expression in cells expressing vector control.
Similarly, the HEK/MRP1 (andC1) and HEK/ABCB1 cells were generated by transfecting the HEK293 with the MRP1 expression vector and the ABCB1 expressing vector, respectively [22].
(A ) qRT-PCR analysis monitoring INK4-ARF expression in WI-38 cells expressing vector or KRAS(G12V).
NB cells were analyzed after transfection with PKCδ expressing vector in order to test the kinase expression pattern.
Cells expressing the mutants did not grow to the same OD600 as cells expressing vector alone, an anticipated effect of protein over-expression.
(C ) qRT-PCR analysis monitoring expression of USP28 and PRKD1 in WI-38 cells expressing vector or KRAS(G12V).
expressing vector.
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