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Results of the four techniques for nocturnal sleep were expressed using as a common measure the sleep efficiency index (SEI) (total sleep time expressed as a ratio of time available for nocturnal sleep) in order to compare them.
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GH30 (previously classified into GH5) endo-beta-1,4-xylanase and GH3 beta-xylosidase are constitutively expressed using G as a substrate.
The unit cost function can be expressed using a ladder diagram, as shown in Fig. 3.
2. Most genes detected as differentially expressed using a regular protocol should also be detected using an amplification protocol.
Genes are identified as being differentially expressed using a number of statistical methods [ 4, 7, 8].
The recombinant plasmid, pET-AprX-SK37 was expressed using E. coli BL21 DE3) as an expression host.
This expressed use prevalence as a percent of the sample.
GAPDH served as an endogenous control, while the leukemic cell line HL-60, in which BAX is expressed, was used as a calibrator, for the normalization of distinct PCR runs.
Unless a stably expressed gene is used as a reference, expression level in samples are overestimated or underestimated, and identification of differential expressions is prone to errors.
A tumour which strongly expressed S100A2 was used as a positive control on all autostainer runs.
TRAP1, identified by GeNorm as one of the two most stably expressed genes was used as a normalizer.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com