In this regard, we sometimes co-expressed two or three genes within one plasmid by placing the genes flanked by the above promoters and inserting transcription termination elements between them, because the selectable marker genes available are limited (URA3, HIS3, TRP1, and LEU2).
The serotypes tested ranged from those that express only a single M protein gene to those that express two or three members of the M protein family.
They might have general reservations but only express one or two specifics.
The Halo software package from Indica Labs, for instance, includes modules for counting cells expressing one or two proteins in the nucleus or cytoplasm, counting cells positive for membrane staining, assessing in situ hybridization, and more.
In any event, our results suggest that adult tissues will be mosaics of cells expressing one or two alleles for some genes.
Here, we report (1) that polygyne workers express one or two forms of GP-9s, which are demonstrated to be general hemolymph proteins unlikely involved in olfaction, and (2) on the identification of a chemosensory protein specifically expressed in olfactory tissues located on the terminal segments (club) of the antennae that house olfactory sensilla.
Among these 125 CT-positive cases, 34 (27.2%) expressed three or more of the eight CT antigens examined.
Compared to wild type wings (Figure 5A), wings that express two or four CG4567-G538E transgenes under the control of ptc-GAL4 exhibit normal proportions but show reduced growth in the area between longitudinal veins 3 and 4 where the mutant proteins are expressed (Figure 5B).
Substantial portions of the miRNAs were expressed under two or three stress conditions.
Only a minor part of genes appeared to be differentially expressed in two or three developmental stages.
In both strains, differentially regulated genes were highly concentrated at the PI and CA phases, and only a small number of genes were differentially expressed in two or three phases.
