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In Sf cells infected with plaque-purified recombinant virus, the cry1Ab gene was expressed to yield a protein of approximately 82-kDa, as determined by immunoblot analysis.
While many autosomal and X-linked genes are dimorphically expressed to yield male and female phenotypes, some sex chromosome-linked genes are expressed solely in one sex or the other and which have essential roles in sex determination.
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We explored whether novel guide strand sequences, such as those based on an siRNA, can be incorporated into the miR-122 short hairpin, expressed and processed to yield functional small RNAs.
Finally, a compilation of community data at WormAtlas (http://www.wormatlas.org) was used to generate a list of 480 genes expressed in bodywall muscle that was further culled of genes also expressed in the hypodermis to yield a more tissue restricted list of 297 bodywall muscle genes.
The DNAs encoding each subunit were joined with different sized linkers and in both possible orders, and expressed in Escherichia coli to yield the corresponding fused protein.
They also sequenced the entire transcriptome (the set of all messenger ribonucleic acid (mRNA) transcripts expressed by the tumour) to yield an additional 6 billion nucleotides.
For trial 160603/902, each reported adverse event in subjects that developed rHuPH20-reactive antibodies was graded mild, moderate, or severe, and the number of adverse events occurring prior to and following the first positive titer were added for these subjects and expressed per time unit to yield an adverse events rate.
The resulting PCR product was used as primers for subsequent site-directed insertional mutagenesis using EEA1 or EEA1-FLAG expressing plasmid as templates to yield in frame N-terminal Ub G76V fusion to EEA1.
We imposed the selection criterion that Escherichia coli cells expressing the P450 had to yield lysate with enough active enzyme to hydroxylate a specified amount of 12-pNCA in 40 min. This criterion roughly corresponds to the case in which an enzyme must catalyze a biochemically relevant reaction at some minimal level in order for its host to survive.
Short hairpins can be expressed and the RNA processed to yield miRNA (miR-122) or siRNA (FLuc).
AID transgenic mice were crossed with hTau mice to yield mice expressing AID transgene, human Tau transgene and one allele copy of mouse Tau.
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