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qRT-PCR was performed for the 60 differentially expressed genes to verify whether their expression levels differed between the ovaries and testes.
Then, scatter plots of expression were analyzed to ensure that probe data within a treatment group were not differentially expressed but also to verify that some probes were differentially expressed between different treatment groups, indicating possible differentially expressed genes.
We used confocal microscopy to examine the HEK293S cells that expressed OR131-2 receptors in order to verify if the expressed OR131-2 receptor is correctly translocated into the cell plasma membrane.
The differentially expressed unigenes were selected to verify the accuracy of the RNA-Seq analysis by qRT-PCR, using the same RNA that was used for RNA-Seq.
All mutations were sequence verified and expressed transiently in HEK293 cells to verify surface expression of the mutated protein.
Then, we used these stably expressing HeLa cell lines to verify that the observed differences in phosphomutant and phosphomimetic DGCR8 protein levels were due to changes in protein stability by measuring protein decay after treating the cells with the translation inhibitor cycloheximide.
The property expressed in formula 2 exists to verify that if a train is given green signal to enter a route, the underlying point(s) present in that route will not change their orientation until the train movement over that route is complete and the routes are unlocked.
For the identification of TCs and GCs, a CYP17A1 antibody, a marker enzyme specifically expressed in TC, was used to verify the purity and identify the cells.
We used whole-cell patch-clamp recordings to verify that expressing AIS-targeted ChR2-YFP-NavII-III in neurons did not affect their fundamental electrophysiological properties.
Quantitative and semi-quantitative real-time PCR were used to verify sex differentially expressed genes as identified from the gonadal transcriptome.
A Crystal core® EvaGreen fluorescence quantitative two-step RT-PCR universal kit (CapitalBio) was used to verify the differentially expressed genes that were identified by microarray analysis.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com