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We constructed biotinylated fusion proteins that linked to three detection tags to GAD65 at the N-terminus, and expressed them in an E. coli expression system.
By complementary application of the omics data, we identified four novel major cellulases from N. patriciarum W5 and expressed them in P. pastoris for functional expression.
If I had reservations, I never expressed them to Jared.
I have instinctively harboured such sentiments myself and occasionally may even have expressed them.
He scrutinised official papers, formed his own views and expressed them.
Such "values" stances were far outside the mainstream of the party before Bill Clinton expressed them.
He was suddenly possessed of opinions, judgments and important questions, and he expressed them.
Mr. Lees had strong, often contentious opinions and expressed them forcefully.
He expressed them through movement, and very much through his outspoken opinions.
His columns brought out the playfulness of mathematical ideas and expressed them in a way the layman could understand.
But social success didn't blunt his populist instincts or the "insane courage" with which he expressed them.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com