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To determine whether this endocytic defect was restricted to the MR or whether other receptors were also aberrantly expressed, the surface expression of other receptors involved in antigen uptake was evaluated.
Analysis of surface antigens by FACS demonstrated that more than 96% of the cells expressed the surface antigens CD90, CD73, CD105 and CD49e.
Moreover, knee MSCs expressed the surface markers CD44, CD13 and CD271 (Fig. 1B), that correspond to the receptor of hyaluronic acid, the membrane alanyl aminopeptidase and the low-affinity nerve growth factor receptor respectively, that have been described to be expressed in MSCs31,32,33.
Single cell suspensions of maternal lung, liver, spleen, bone marrow, and blood were analyzed between late gestation (day e16 18) and 1 day post-partum for the number of GFP-positive fetal cells in relation to 107 maternal cells and the percentage of GFP-positive cells that expressed the surface markers CD11b, CD29, CD34, CD44, or CD105.
MΦ in both strains expressed the surface markers F4/80 and Ly6C, suggesting an inflammatory phenotype [15] (Figure 4A).
Recently, we transiently expressed the surface antigen 1 (Sand) and granule dense 4 (GRA4) of Toxoplasma gondii in tobacco leaves [ 13, 14].
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All MSC express the surface markers CD29, CD73, CD90 and CD105 and they lack the expression of CD14, CD31, CD34 and CD45 [ 14, 51].
Cultures in rotating wall vessel bioreactors and orbital shake flasks with intermittent shaking both resulted in an elevated multiplication of cells expressing the surface marker CD34+ that labels hematopoietic stem and progenitor cells (HSPCs)—compared to static cultures51, 52.
The constant values derived from adsorption isotherm could express the surface properties and affinity of adsorbent.
An adsorption isotherm is usually characterized by certain constant values, which express the surface properties and affinity of the adsorbent.
NKT cells are also found in cord blood [5], [6] where they already express the surface markers associated with activation.
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