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The ability of the expressed proteins to form channels was assessed by dual voltage clamp electrophysiology [28] of cell pairs in which one cell expressed ShakB(N+16) and the other ShakB(L) (heterotypic) or both cells of a pair expressed the same protein (homotypic).
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However, unlike wild mice, different individuals from the same strain express the same protein pattern, an artifact of many generations of inbreeding.
For instance, cMyc oncogene when expressed induces proliferation but suppresses cancer metastasis [ 169], i.e., negative and positive roles played by over-expressing the same protein.
However, expressing the same protein in HeLa cells, causes an increase in apparent molecular weight and the native protein detected by specific antibodies in differentiating muscles cells runs even higher.
In a murine CLP model, treatment resulted in a decrease in lymphocyte apoptosis, but the effect was less marked than that observed in transgenic animals constitutively expressing the same protein (30 ).
Three gene fragments [N1 (1–1269 nt), N2 (1 327 nt) and N3 (328–1269 nt)—expressing the same proteins of N1, N2 and N3, respectively] of SARS-N were cloned into pVAX-1 and used to immunize BALB/c mice by electroporation.
They are important during development and for keeping tissue integrity and morphology, since cells expressing the same proteins stick together.
(D ) Correlation between the fluorescence recovery in the bleached bud compartment (t15) of the depicted proteins fused to GFP and the pf in cells expressing the same proteins fused to TetR.
To scrutinize whether dual localization in Arabidopsis is an artifact by overexpression of DMP1-eGFP by the CaMV 35S promoter, we expressed the same fusion protein from the native DMP1 promoter in transgenic plants.
While Myc-tagged ribosomal protein was abundantly expressed and was ubiquitously distributed in mutant parasites expressing RPS13, the same protein was scantily expressed in parasites with knocked-down RPS13 (Figure 4B).
In contrast with the original Physarum NOS form A cDNA library clone (AF145041) that still contained 104 additional amino acids 5′ to the putative start methionine [ 18] and a variant A1, which contained only 29 amino acids 5′ to the putative start, the clone starting at the predicted methionine (A2) had no enzyme activity, although it expressed the same amount of protein.
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