Regardless of whether intracellular or secreted transgenes were expressed, the regulation factors of all the tested cell lines were between 10 and 400.
Among these 5 miRNAs, miR-20a and miR-20b were validated as regulatory miRNAs in hypoxia-induced VEGF expression, whereas the other three could be expressed for the regulation of other genes without over-repressing VEGF expression due to competitive action.
Since HSPs are expressed under the regulation of HSFs at transcriptional level (Liu et al. 2013c), the above 5 up-regulated HSP genes might be supposed as target genes of HsfB3 under As stress in P. notoginseng.
As he wrote in The Economic Consequences of the Peace, "The preservation of a spurious value for the currency, by the force of law expressed in the regulation of prices, contains in itself … the seeds of final economic decay, and soon dries up the sources of ultimate supply".
In this assay GFP is expressed under the regulation of the sulA gene promoter, an SOS-network gene [48], and GFP fluorescence is thus an indicator of SOS induction.
- Similarly, homeostatic synaptic plasticity is expressed via the regulation of AMPAR synaptic accumulation.
EBF1 and EBF2 were each expressed under the regulation of the constitutive CAULIFLOWER MOSAIC VIRUS 35S promoter in ecr2-1 ctr1-10 and ctr1-1.
More definitive data to evaluate the role of GLI1 in tumour initiation and its relationship to NRP2 were obtained using a transgenic mouse model of breast cancer in which GLI1 is expressed under the regulation of the MMTV promoter (Fiaschi et al, 2009).
We investigated the possible roles of these differentially expressed miRNAs in the regulation of gene expression using a cell-based model.
To circumvent this problem, we expressed LacZ under the regulation of BP4 in mice and demonstrated, for the first time, that human BP4 is active in brain structures that express BDNF mRNA and are known to be altered in MDD and BD (10 13,63,64).
