Sentence examples for expressed the constructs from inspiring English sources
We expressed the constructs in 293T cells, immunoprecipitated the proteins with anti-Flag-antibodies and subjected the immunoprecipitates to an in vitro kinase assay with recombinant Zz-tagged Plk2.
GM prepared and expressed the constructs in E. coli, and made bacterial preparations.
We expressed the constructs in HEK-293T cells, isolated mitochondria and analysed them by BN-PAGE and Western blot.
We therefore exchanged the last 12 amino acid residues of PorB Ngo with those of OmpC Eco and vice versa, and expressed the constructs named PorB-12aaOmpC and OmpC-12aaPorB in HeLa cells.
These effects are considerably more dramatic than previously reported (Harel et al., 2008), presumably because we expressed the constructs at lower than endogenous levels so as not to saturate the intracellular sorting machinery.
The next day, 9 μg/mL puromycin was added to the medium to select for cells that had integrated and expressed the construct.
UML (Unified Modeling Language) is a mechanism for expressing the constructs and relationships of complex systems, in particular software systems.
Cells were allowed 24 h to express the constructs before the caspase assay was performed.
We observed, however, that the numbers of cells expressing the constructs differed between the KChIPs.
