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We expressed the construct in the intestine.
All plants that expressed the construct exhibited the phenotype presented here.
The next day, 9 μg/mL puromycin was added to the medium to select for cells that had integrated and expressed the construct.
For most experiments, the cells were stably transfected with HA-tagged wild-type or mutant VAMPs (2, 3, and 8), and cell lines were selected that expressed the construct at low levels so as not to saturate sorting machinery.
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Cells positive for c-Myc (i.e. expressing the construct) were analysed for γH2AX foci.
Stable BY-2 cell lines expressing the construct H 6-GFP-BD-CVIL were previously reselected for good fluorescent intensity.
(A – B ) Drep-2C-Term and BrpNc82 staining in animals expressing the construct mb247::Dα7GFP that marks acetylcholine receptors in MB KCs.
Expression of the RAP1 – RIfusionfusinn in tlc1 Δ had little effect on telomere length, while tlc1 Δ rif2 Δ cells not expressing the fusion construct had slightly longer telomeres than those expressing the construct.
For example, Wyttenbach et al. developed a tetracycline (Tet -ON-inducible PC12 cell line Tet -ON-induciblence of Tet -ON-inducibleycline, exPC12ses the celltruct at linelevels.
Results were normalized to the endogenous GAPDH (glycerinaldehyd-3-phosphatdehydrogenase) control and compared with a reference sample (cells expressing the construct wt-GLP-1R).
Only 20% of the total number of transgenic lines that express the construct showed α-glucuronidase activity above the WT level (Additional file 2: Figure S2B).
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