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Interestingly, the chemokine CXCL14 is the highest expressed taste-bud associated gene in our database.
Similar expression patterns for anti-mouse GAT4 were found in the GAD67-GFP expressing taste cells (Figure 10).
We also used a transgenic mouse line that expresses GFP in GAD67 expressing taste cells which identify a subset of Type III taste cells [24], [25].
Some immunoreactivity overlapped with the IP3R3-GFP expressing taste cells (Figure 6A D, see arrowheads) while other IP3R3 expressing cells (see arrow) were not immunoreactive for GABAB1.
We also used transgenic mice that express green fluorescent protein (GFP) in either the Type II taste cells, which can respond to bitter, sweet or umami taste stimuli, or in the Type III GAD67 expressing taste cells.
Since gustducin is found in a subset of PLCβ2/IP3R3 expressing taste cells [78], we predict that the GABAB2 labeling is likely present in the Type II taste cells that do not express gustducin.
Since GAD67 expression has been localized to Type III taste cells in mice [24] and both Type III and gustducin expressing taste cells in rat [12], the presence of the GABAA receptor on some Type II cells make them a potential target for modulation by other taste cell populations in the taste bud.
However, since its expression in other cell types has not been rigorously characterized and electron microscopy studies have demonstrated that almost all IP3R3 expressing taste cells are Type II cells [57], for this study we are presuming that the presence of IP3R3 identifies the taste cell as a Type II cell.
As a control for the methodology, we also tested several non-GFP expressing taste cells.
We conclude that PLC expressing taste cells do not communicate with the nervous system via conventional synaptic mechanisms.
GFP expressing taste cells that did not show a Ca2+ response to m-3M3FBS were eliminated from further analysis.
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