Differentially expressed clones spanning a single ORF were used for the identification of differentially expressed genes and the other clones were used in order to confirm or reject data obtained.
When compared to non-malignant CD19+ B cells from healthy donors, the CLL histone proteome showed a distinct signature of differentially expressed species, spanning all the histones studied and including both post-translationally modified species and unmodified, non-allelic replication-dependent histone isoforms.
Estimates of genome coverage were not possible as no sequenced genome is available for Polykrikos lebouriae or any species within its more inclusive clade (i.e., the Gymnodiniales).> SLPCR amplified a diverse array of nuclear transcripts from P. lebouriae (Fig. 2), suggesting that the cell expressed genes spanning a broad range of functions, including photosynthesis.
To design primer sets, we first BLASTed our fragments against Lactuca or Helianthus ESTs and then used the "hit" ESTs for primer design because the ESTs are likely longer than the isolated fragments, with a greater chance that the expressed sequence spans the introns in genomic DNA.
A total of 28 weakly expressed chromosomal regions, spanning 408 Mb in total, were identified in CD34+ cells.
DOI: http://dx.doi.org/10.7554/eLife.10308.008 To define the region of HERC2 that recognizes NCOA4, we sub-cloned and expressed six fragments spanning the HERC2 open reading frame as depicted in Figure 3B (Bekker-Jensen et al., 2010).
The highly inducible NF-ATc1/A contains a relatively short C-terminus whereas the longer, constitutively expressed isoform NFATc1/C spans an extra C-terminal peptide of 246 amino acids.
The data showed that, whereas, keratinocytes mainly express CerS1-1 mRNA (Fig 6B), or CerS1-2 conthening the A1-polyA sequence (Fig 6C, upper panel, lanes 1 and 2 3, respectively), HNSCC cells express CerS1-2 mRNA spanning the A5-polyA sequence (Fig 6C, middle panel, lanes 2 3 and 1, respectively), which contains the miR-574-5p-target miR-574-5p-target miR-574-5p-target miR-574-5p-target
Furthermore, Dittmar et al. reported that isoaccepting tRNAs are differentially expressed in a study spanning eight human tissues [ 25].
GMAP [ 36] and manual analyses were performed using sequenced PCR products obtained in this study, as well as expressed sequence tags (ESTs) spanning at least one intron.
