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Thus, Prdm1 was expressed in myogenic cells prior to terminal differentiation; and, after differentiation, Prdm1 expression was not limited to cells that expressed slow myosin heavy chain isoforms.
Thus, Prdm1 was expressed in myogenic cells prior to terminal differentiation and expression was not limited to cells that expressed slow myosin heavy chain.
In fetal cultures, as in embryonic cultures, therefore, Prdm1 expression was not limited to cells that expressed slow MyHC s), but was also found in myotubes that expressed only fast MyHC s).
Thus, Prdm1 was expressed in chicken myogenic cells prior to terminal differentiation and, after differentiation, Prdm1 expression was not limited to cells that expressed slow myosin heavy chain isoforms.
Because none of the three slow MyHC isoforms is expressed by fast myotubes in E4 cultures [31], this result showed that Prdm1 expression was not limited to differentiated cells that expressed slow MyHC, but was also found in myotubes that expressed only fast MyHC s).
We found that Prdm1 was expressed in all of these different types of myogenic cells and was not limited to differentiated muscle cells that expressed slow MyHC.
Similar(54)
APs in these neurons typically express slow, canonical onset dynamics.
In addition, expression of zebrafish Prdm1 is limited to a subset of all of the myofibers that express slow MyHC s), whereas we found that chicken Prdm1, at least in cultures, was expressed in all myotubes, including those that did not express slow MyHC s) such as the embryonic and fetal fast myotubes.
Fetal myoblasts form large multinucleate myotubes that initially express only fast MyHC s), though, after 7 10 days of differentiation, a percentage of the fetal myotubes begin to co-express slow MyHC1 (but not slow MyHC2 or 3) along with the fast MyHC s) [31].
Furthermore, the numbers of developmental MHC fibers were less than in affected TC muscle, and almost all of these fibers co-expressed slow type MHC, unlike TC muscle.
In regenerating fibers of the CXMDJ diaphragm, the proportion of myofibers expressing slow type MHC increased markedly after 4 months old (Fig. 6C).
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