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The microarray analysis showed that in the cultured myotubes compared to the tissue biopsies 1260 transcripts, which correspond to 1216 nuclear genes, were differentially expressed (selection criteria: absolute fold-change value > 2 with a corrected p-value of p < 0.01).
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Constitutive promoters are usually also applied to express selection markers.
For this, TaqMan Low Density Arrays using 384-well micro fluidic cards were pre-loaded with a selection (Table 3) of differentially expressed genes (selection was primarily based upon the availability of standardized primer pairs to quantitatively investigate respective gene expression).
Many applications such as highly expressed cell selection and various single-cell analysis would be applicable.
In the words of Brown biochemist Kenneth Miller: 'As any student of biology will tell you, because those genes are not expressed, natural selection would not be able to weed out genetic mistakes.
Expressed gene selection proceeded from the array database as outlined in Figure 1.
In contrast, genes considered late, such as alpha-myosin heavy chain (α-MHC) and TNNI, were highly expressed after selection.
All analyzed calluses expressed both selection marker and (except for those with an empty vector) the corresponding bp100der transgenes (Additional file 1).
Our work has shown that the same Illumina BeadChip data from published experiments churns out better differentially expressed gene selection after applying our proposed summary statistic.
While fears have been expressed that selection for bTB resistance will reduce sensitivity to SICTT testing, it is thought that such a scenario will not arise.
H3GA0016037 lies between the gene encoding transcription factor NEUROD4 neurogenic differentiation 4 and the TESPA1 thymocyte expressed positive selection association 1 locus.
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