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Exact(8)
The BAGEL analysis procedure sets the lowest expressed sample to 1 so that the expression units on reaction norm plots represented fold change relative to the lowest measured sample.
As requested by the software, Ct values were converted to linear expression quantities by 2-ΔCt using the highest expressed sample as calibrator.
For the TaCKX gene family, TaCKX1 was the most highly expressed family member, with expression from before anthesis through to14 daa and relative expression levels of 220 to 980-fold higher than that in the lowest expressed sample.
In older leaves, while other ZOG genes expressed at very low levels, there was a sharp increase of mRNA for both TacisZOG1 and TacisZOG2-1, Thexpressionof of TacisZOG1 was over 900-fold higher than that in the lowest expressed sample tested and was particularly high from 21 daa, when the chlorophyll content in the basal one-third of the leaf started to decrease.
The geometric mean of the Ct value of the endogenous control genes was used to normalize the data and the lowest expressed sample was used as a calibrator.
Subsequently, technical replicates were averaged and relative quantities (fold changes, FC) were calculated based in the less expressed sample for each assay.
Similar(52)
A first approach that used metabolite mass spectrometry data of multiple expressed samples was introduced by Fiehn et al[224].
(F) Mammol/Lalian cell (293T cell) expressed EBOV NPΔ451 739 protein purified following the same protocol as described above for E. coli expressed samples.
False discovery rate was estimated based on the probability of encountering the observed number of differentially expressed samples in the treated group, given the total number of observed differentially expressed samples in both groups, under the empirically determined distribution derived from the null hypothesis that the differentially expressed samples are equally distributed in both groups.
As above, NTC did not amplify, and -RT controls were more than 6 Ct above the lowest expressed samples.
Given that DI is produced in bacteria however sufficient measures should be undertaken to ensure expressed samples are free from lipopolysaccharide.
Related(20)
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