Figure 2D shows that all the tested human breast cancer cell lines (MDA-MB-231, Hs578T, SK-BR-3, T-47D, and MCF7) expressed relatively low levels of α9 β1 integrin in comparison with 468LN.
Of these, SPC-A1 and NCI-H1975 expresignificantlyantly higher levels of HOTAIR compared with the normal bronchial epithelial cell line (16HBE), while A549 cells expressed relatively low levels of HOTAIR.
Of these, four cell lines (SPC-A1, NCI-H1650, NCI-H1299 and SK-MES-1) expressed higher levels of miR-196a compared with the normal, bronchial epithelial cell line, 16HBE, while A549 cells expressed relatively low endogenous levels of miR-196a.
Rather, these results implicate a role for SNARK in other tissues, particularly since skeletal muscle expresses relatively low levels of SNARK compared with liver, kidney, intestine and testis in rodents [ 11].
SK-MEL-28, a melanoma cell line considered to express relatively low levels of MITF [18], clustered with the lower MITF Motif 1 cell lines as expected (Figure 1A).
Western blot analysis of the two strains in this panel that are homozygous for the variant allele revealed that they express relatively low amounts of DT-diaphorase protein, consistent with the role of the substitution in protein instability.
We initially confirmed that the control ST cells do express relatively low levels of endogenous pAPN.
JEG-3 cells express relatively low amounts of TFAP2A.
These cells express relatively low basal levels of endogenous c-CBL (data not shown).
