Exact(22)
In both batches, an high level of protein expression has been obtained after only four hours post-induction and no expressed product were observed before induction.
Monoclonal antibodies specific for native MOMP reacted to the expressed product by immunoblot.
Surprisingly, we found that SGSH was poorly secreted from cells, resulting in retention of the expressed product.
The synthetic gene was cloned into a T7 promoter-controlled plasmid (pET-3a) and the expressed product was analyzed to assess antigenicity, cellular localization and function.
However, the expressed product did not show any NHase activity when the downstream activator gene was ignored, and the product completely existed in insoluble inclusion body.
In addition, we used the PSB1 and primer 5'- GCGAGCACAGAATTAATACGACT-3', which is complementary to the adaptor sequence to amplify the expressed product of nuclear psb28.
Similar(38)
The expressed products associated with probes indicating differential expression were confirmed by qRT-PCR.
Subsequently, HqL07 and HqL09 were expressed in vitro and the molecular weights of the corresponding expressed products were 60 and 70 kDa, respectively.
The expressed products possess native-like properties.
In recent years, a number of genes or proteins have been identified through analyzing the expressed products of genes after F. graminearum infection [37] [39], [67] [71].
Recent studies have indicated that the H. parasuis vtaA genes reveal diversity and the expressed products are good candidate immunogens [49], [50].
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