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The result of LC MS/MS combined with chemical tagging method was validated by confirming the expression and localization of one of the differentially expressed plasma membrane proteins, CD43, by FACS and confocal microscopy.
Next, four differentially expressed plasma miRNAs were chosen to validate their expression in other large scale samples by real-time PCR.
Ontology groups and associated differentially expressed plasma proteins in the time points of 8 h after HA versus HA-sham treatment.
Twenty-three diffexpressedy explasmad proteinsroteins were identified, demonstrating the effectiveness of the described protocol and defining a set of candidate biomarkers to be validated in independent samples.
The differentially expressed plasma membrane proteins were analyzed between CML cell line (K562) and APL cell line (NB4) using the comparative proteomic approach.
By contrast, a fold change ≤0.83, P < 0.05 represents less protein abundance in T0 or 8 h versus baseline Fig. 5 Differentially expressed plasma, BALF and lung proteins identified with iTRAQ in LPS + HA (sham)- versus LPS + HA-treated pigs.
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HL60 cells express plasma membrane-associated NADPH oxidase [32] that can generate a superoxide radical (O2-).
Marrow examination showed 14% monotypic lambda expressing plasma cells.
10.7554/eLife.01936.012 Figure 7. Midbrain DA neurons express plasma membrane GABA transporters.
Approximately the same frequencies of IgA expressing plasma cells were noted in the interstitial areas of all three groups tested.
We express plasma PTX3 and CRP levels, ESR and other scalar variables as medians values and ranges.
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