To directly test the role of RYBP in maintaining levels of H2AK119u1, we analyzed Eed +/+ and Eed−/− mESCs in which we expressed one of two different shRNA hairpins to deplete RYBP levels.
We first introduced GFP into the 4T07 cells for visualization and then generated stable 4T07 pools that expressed one of two different APC-specific shRNAs, a scrambled shRNA (SHC-002) or empty vector (pLKO.1).
To elucidate the role of heterogeneity in receptor expression in the observed synergy between an EI and a drug of another class, we constructed a conceptual model where target cells in culture expressed one of two discrete levels (high or low) of one entry receptor and were identical otherwise.
Participants were told to state whether faces expressed one of six possible emotions (happiness, anger, disgust, fear, sadness, neutrality) in two sessions, one with upright faces and the other with upside-down faces.
Most P. aeruginosa isolates express one of two types of flagella (A or B) based on the deduced amino acid sequences of the major structural gene fliC, encoding the flagellin filament [ 116].
To confirm the specificity of these findings, we next generated MDCK cell clones stably expressing one of two shRNAs directed against ANO6 (shand6#1 and shANO6#2) or control shRNA (shCtrl), respectively.
To determine the importance of SteC Lys in F-actin meshwork formation by Salmonella in infected cells, Swiss 3T3 cells were infected with an steC mutant strain expressing one of two epitope-tagged versions of SteC (SteC-2HA or SteCK256H-2HA) from a plasmid.
MDAMB-231 cells expressing one of two distinct shRNA, shIPS-1-A or shIPS-1-B, were generated, and these cells showed a marked reduction in endogenous IPS-1 compared with the non-specific shRNA-transfected cells, as shown by immunoblot and confocal microscopy using an anti-IPS-1 antibody.
In the EDT condition, participants were presented with a triplet of faces expressing one of seven emotions (anger, disgust, fear, happiness, sadness, surprise, or calmness).
53 Another study in 1800 healthy infants showed that when administered together with routine vaccines, a primary immunisation course of 4CMenB is immunogenic against three reference strains expressing one of three vaccine antigens.
Amino acid sequences for candidates, queries, and outgroups are found in Additional file 6. (PDF 8376 kb) Additional file 22: Immunoblot analysis revealing the presence of His-tagged recombinant protein in soluble extracts of Escherichia coli strains expressing one of six N -methyltransferase candidates (GFLNMT1-6) from Glaucium flavum.
