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However, it is likely that this number is an underestimate, because expression profiling studies will miss target genes whose expression patterns are altered by Hox regulation, but are still expressed, on average, at similar levels in both tissues.
Mapping tags are expressed on average at 50.78 cpm, while non-mapping tags only at 19.85 cpm.
Protein-coding mRNAs are expressed on average at a much higher level compared to non-coding transcripts.
Estimates from various analytical and cell-based techniques also indicate that individual peptides are expressed on average at 50 copies per cell with extremes ranging from 1 to 10,000 copies per cell (Granados et al., 2015).
Further on, the relative quantification of these AS events is similar in breast tumor and blood samples, with Δ1Aq expressed on average at similar levels than the full-length reference and Δ8p, Δ9,10 and Δ14p, signals representing roughly 35%.
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Oocytes expressed on average 8,728 genes.
Awi-Spherulin is expressed on average 20 times higher than the previously characterized spherulite-forming gene Astrosclerin [ 7], and at almost 28% the level of actin, indicating it is a highly expressed gene.
The RT-qPCR analysis revealed that all five are expressed at statistically significantly higher levels in SA24T0 than EC24T0 samples, including ABCG2 which was expressed on average 20.9 fold higher levels in SA24T0 than EC24T0 samples.
All tissues examined expressed, on average, more full-length isoform 2 than the shorter isoform 3.
While the time to onset of detectable serum levels appeared the same for all serotypes, types 1, 3, and 5 produced 100- to 1000-fold more cFIX than type 2. In fact, 12 weeks after transduction, type 1 continued to express levels of cFIX on average at 80 μg/ml followed by type 5 (6.52 μg/ml), type 3 (3.27 μg/ml), type 4 (258 ng/ml), and finally type 2 (90 ng/ml).
We have described the production of large LongSAGE and MPSS libraries from a single RNA sample and consider their usefulness for identifying the complete transcriptome of a clonal population of cells, including transcripts not expressed in all cells and hence present on average at less than one copy per cell.
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