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The amount of angiopoietin protein in placentas was quantified by densitometry using Scion Image 4.0.3.2 (Scion, Frederick, MD) and expressed normalized to the density of the actin band.
Data are expressed normalized to the average of the relevant control group.
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Gene expression data are expressed normalized to GAPDH and as a fold change of the mean of the day 0 condition.
Gene expression data are expressed normalized to RNA18S5 or GAPDH and as a fold change of the mean of the healthy controls.
Data are expressed normalized to GAPDH or HPRT as fold-change of the mean of the control group.
Bladder MIF and SDF-1 levels are expressed normalized to protein amount (BCA, Thermo Scientific) present in the samples.
Data are expressed normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) expression and as a fold change of the mean of the controls.
The resulting fluorescence signals, F, were expressed normalized to their baseline values F0.
Data are expressed normalized to either RNA, 18S ribosomal 5 (RNA18S5) or glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene expression and as a fold change of the mean of the controls.
All M-gene quantities are expressed normalized to B-actin level.
The target gene expression was calculated relative to values from glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and is expressed normalized to pretreatment values.
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