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Therefore, we explored the signalling molecules that induce CD4+ TSCM cells, and we subsequently discovered that Notch signalling converted activated CD4+ T cells into TSCM-like cells, which expressed naive markers, CD44loCD62Lhi.
(2014) reported that PSC cultured in a cocktail of six kinase inhibitors plus LIF and activin (6i/L/A) on feeders expressed naive features.
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Both TSCM and TMNP cells, which express naive T cell markers (CD45RA+CD45RO−CCR7+CD62L+), respond rapidly to antigens, express multiple effector molecules and produce memory and effector cells.
Under these conditions, we saw no differences between the three conditions compared with cells expressing naive levels of Drp1.
(B – G ) Quantification of the synaptic accumulation of BCRs in J558L cells expressing memory B1-8-IgG-BCR (B ), J558L cells expressing memory B1-8-IgG-BCR equipped with a mIgM cytoplasmic tail, termed GGM (C ), J558L cells expressing naive B1-8-IgM-BCR (E ) and J558L cells expressing naive B1-8-IgM-BCR equipped with a mIgG cytoplasmic tail, termed MMG (F ).
(A, B ) Statistical quantification of the synaptic recruitment of IgM-BCR in J558L cells expressing naive B1-8-IgM-BCR (and and primary naive B cells expressing B1-8-IgM-BCR (B ).
(B ) Statistical comparison of FI of hundreds of BCR microclusters in the immunological synapse in J558L cells expressing naive B1-8-IgM-BCR encountering NP-TGT sensors with indicated tension force.
(E ) Quantification of the synaptic accumulation of IgM-BCRs in J558L cells expressing naive B1-8-IgM-BCR to show that non-specific NP-TGT that were tethered on coverslip in a neutravidin-independent manner cannot activate B cells.
J558L cells expressing naive B1-8-IgM-BCR weitherther placed on neutravidin-coated coverslip that were incubated with 56 pN NP-TGT sensor or placed on neutravidin-non-coated coverslip that were incubated with indicated types of NP-TGT sensors.
(D – F ) Statistical comparison of the volume of pSyk (D ), pPLCγ2 (E ), or pTyr (F ) microclusters in J558L cells expressing naive B1-8-IgM-BCR that were produced by 12 pN, 43 pN, or 56 pN NP-TGT molecules.
(D, E ) Comparisons of averaged traces showing the dynamic accumulation of naive IgM-BCRs into the immunological synapse (D ) and the growing features of the size of contact area (E ) for J558L cells expressing naive B1-8-IgM-BCR as demonstrated in (C ) in a 10 min TIRFM imaging time course.
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