During alcoholic fermentation of sugars, the glycolytic genes are the most efficiently expressed genes in yeast, and glycolytic enzymes comprise over 30% of the soluble cell protein [ 28].
Results showed that soluble Active-H was most efficiently expressed in the pET-28a vector-transfected Rosetta (DE3) bacteria, after treatment of 0.1 mM of IPTG at 17°C for 24 hr.
Witte and co-workers demonstrated that endogenously expressed GH levels were most efficiently labeled by the epoxide probes, and that a two-step click chemistry labeling approach can be employed, but careful probe design must be made or the two-step approach is poor [ 29].
While far from perfection, the LC may have evolved to this dissociated stage to express its protease activity most efficiently.
As in U-2 OS cell lines, dexamethasone (DEX), a potent glucocorticoid, induced apoptosis most efficiently in Jurkat cells expressing the GR-C3 isoform, whereas the GR-D3 isoform-expressing cells were resistant to glucocorticoid killing.
White light activates M cones, rods, and melanopsin, whereas UV light is most efficiently absorbed by UVS opsin (expressed in M cones and S cones).
As expected from the assays using purified proteins, lysates from cells expressing active caspase-2 cleaved Ac-VDTTD-AFC more efficiently than the other peptides, and extracts from cells expressing active caspase-3 cleaved Ac-DEVD-AFC most efficiently.
It is well established that highly expressed genes have strongly biased usage of alternative synonymous codons and that of preferred codons, which are thought to be translated most efficiently by the most abundant tRNAs, and the lowly expressed genes have less biased codon usage patterns.
The co-IP of these PDE4D splice variants expressed exogenously in HEK293 cells identified PDE4D8 as the variant that most efficiently interacts with β1AR.
We showed that an authentic RNA retroviral genomic platform was most efficiently transferred but that adding UTR sequences from highly expressed genes could improve expression upon transfection while having only a slight effect on expression from transferred RNA.
