For this purpose, we generated a set of mammalian suppression/re-expression plasmids that knocked down endogenous GSK-3 β and expressed modified GSK-3 β that contained either a nuclear export signal (NES) for cytoplasmic targeting or a nuclear localization signal (NLS) for nuclear targeting.
The amplicon vectors were packaged using a gC−, lacZ+ helper virus to generate a mixture of high-titer helper virus (lacZ+) and amplicon vectors (GFP+), which expressed modified gC in the virion envelope.
These data suggest that SUMO-deficient PRs are important mediators of increased cell proliferation and pro-survival signaling; cells expressing modified PRs undergo biological processes consistent with their associated gene expression profiles.
To increase exon 7 inclusion of SMN2, we have designed a series of vectors that express modified U7 snRNAs containing antisense sequences complementary to the 3′ splice site of SMN exon 8.
Nevertheless, one could expect an increased circulation of isolates expressing less or no vaccine antigens, i.e. less virulent ones or expressing modified isolates.
If recombination occurs between specific regions of the YFP and CFP genes these HIV-1 recombinants will express modified Green Fluorescent Protein (GFP*) which can be observed by FACS analysis [14].
In this study, we tried to detect the central functional domain(s) of GCS1, using complementation assay of ArabidopsisGCS1 mutant lines expressing modified GCS1.
To make the U7 snRNA methodology more amendable for gene therapy approaches, we generated Adenovirus type 5-derived vectors that constitutively express modified U7 snRNAs.
Recent studies performed in non-human primates receiving an intravitreal injection of an AAV vector expressing modified soluble VEGF receptor (sFLT01) have observed a reduction in laser induced CNV [40].
These constructs express modified Flag-Sox2 proteins: Flag-Sox2-HMG that contains only the DNA binding domain, Flag-Sox2- 1-123) Flag-Sox2- 1-123e N-thatinal and the DNA-binding domains, or Flag-Sox2-ΔHMG that lacontainsDNA binding domain (Figure 2A).
For example, direct induction of apoptosis by expressing modified caspase-8 [ 1, 2], caspase-3 [ 3], or caspase-9 [ 4] under cell type-specific promoters is well established.
