In this study, we isolated a mutant (named hip3-1) that expresses Hip3 missing its C-terminus (Hip3-1).
Transplantation of autologous HSCs genetically modified to express the missing ALD protein may circumvent these severe complications (Fig. 2).
In all cases, growth of the mutant strain expressing the missing gene in trans was indistinguishable from that of the wild-type, thus ruling out polar effects.
To further investigate the role of miRNAs in modulating lifespan, we analyzed the effect of expressing the missing miRNAs directly in the miRNA mutants.
Some studies used transient transfection to re-express the missing caveolin-1 and test for its function in signaling [ 7, 39].
We reasoned that the clearance of circulating H2S, by expressing the missing ETHE1 gene in a filtering organ such as the liver, could decrease the levels of H2S, thus acting as a detoxifying means for effective disease treatment.
Whatever the mechanism of this extraordinary CNS correction, it is likely that many laboratories will try to replicate these studies with AAV vectors expressing enzymes missing in other lysosomal diseases affecting brain (Krabbe disease, Batten disease, Tay-Sachs disease and many others).
The lack of information resulted in anxious posts on the Confetti chat forums as worried wedding planners expressed concern over missing items ranging from bridesmaid's dresses to purple topiary trees and personalised chocolates.
Though more than excited to be back in the Race, Mattel expressed disappointment at missing three challenges.
Commissioner Connor expressed regret at missing seeing Shuttlesworth get hit and said he "wished they'd carried him away in a hearse".
Jezebel writer Kelly Faircloth urged the retail giant to "fuck off!" Target has since expressed regret for missing the mark with its corporate tribute—"we sincerely apologize if some event-goers felt it was not the best way to capture the spirit of the neighborhood," the company wrote in a statement.
