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Mesp2 was weakly expressed at E6.5 7.0 in a pattern very similar to Mesp1 expression, but expressed longer in Mesp1 embryos compared to wildtype.
Correspondingly, PC-12 cells grown on PP films that had been previously adsorbed with FN during immediate stimulation expressed longer neurites.
B6 subjects expressed longer freezing responses than BALB subjects after exposure to objects receiving the CS-UCS contingency (Fig. 3a; effect of subject genotype, F[1,124] = 53.8, P<0.0001).
B6 subjects again expressed longer freezing relative to BALB subjects (Fig. 3c) in response to presentation of the CS-only (orthogonal contrast, P = 0.003) and the paired CS-UCS (orthogonal contrast, P<0.001).
Even after pre-exposure to objects experiencing this unpaired CS-UCS, B6 subjects expressed longer freezing responses than BALB subjects (Fig. 3b) during several of the CS-only (orthogonal contrast, P = 0.03) and paired CS-UCS (orthogonal contrast, P<0.001) test trials.
When test mice were assessed during the first 28 s of a CS that was forward paired with a UCS (the UCS was a 2-s shock) for 10 consecutive trials, test mice from both strains expressed longer freezing responses across successive conditioning trials (Fig. 2b).
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One thing Twitter didn't measure was the use of threading, which seems to be the more popular way today of expressing longer thoughts.
Finally, a fourth challenge may be the apparent difficulty of expressing longer scaffoldin variants.
Investigating the protective effect of calpain inhibition in other HD models (e.g., those expressing longer Htt variants) may therefore be of value.
In this study we have explored the feasibility of expressing longer peptides (>40 amino acids) of the E2 protein fused to the coat protein of PVX via 2A peptide from FMDV (Foot and Mouth Disease Virus).
In particular, S. flexneri has been shown to be primed for invasion in anaerobic conditions, in which it expresses longer T3SS needles while reducing Ipa (invasion plasmid antigen) effector secretion; this results from FNR-mediated repression of the virulence plasmid genes, spa32 and spa33[ 7].
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