In SKOV3, MAGE-1 was expressed (line 2, a band ranging between 400-500 bp).
Secondary changes in gene expression in the constitutively expressing lines may mask CES and CES SRDX primary effects.
These lines were then screened for Pen-2 expression, and where possible, equally expressing lines were selected.
In addition, GmSKRPs associate with a complex that contains key splicesome components and more than four hundred genes were differentially spliced in GmSKRPs and PsAvr3c expressing lines.
The level of unesterified astaxanthin reached ca. 14 μg g−1 DW in some bkt1 expressing lines of S. phureja but was much lower in the S. tuberosum background.
However, methylation in a single SmaI site at position-69 was observed in all MGMT deficient lines but not in any MGMT expressing lines.
Stably expressing lines were created per manufacturer's protocol.
This was true also for all the (CUG 240 expressing lines but one, (CUG 240.4.
As a consequence, high expressing lines are counterselected and tuber carotenoid levels show only modest (up to 3-fold) increases.
Two δOAT-GFP expressing lines had 8.5 and 20.4-fold higher OAT activities than the wildtype.
In contrast, no increased root hair elongation was observed in the ARR1 expressing lines.
