Bacteria hemoglobin from Vitreoscilla stercoraria — Vitreoscilla hemoglobin (VHb), when expressed in various hosts in oxygen-limited conditions, has been shown to improve growth, protein secretion, metabolite productivity and stress resistance of hosts, thus rendering the protein promising in metabolic engineering, especially in plant metabolism optimization.
Only a small number of bacteria express hemoglobin proteins and the best studied of these is from Vitreoscilla sp. Vitreoscilla hemoglobin (VHb) has been expressed in various heterologous hosts under oxygen-limited conditions and has been shown to improve growth and productivity, rendering the protein interesting for biotechnology industry.
The newly developed online tool forms a reliable means for identification of alternatively spliced transcript-isoforms that may be differentially expressed in various tissues, cell types or physiological conditions.
To obtain a broad representation of genes expressed in various tissues under normal and stressed conditions, we used gill, liver and brain tissue that had been collected from a total of ten different fish acclimated to either −1.5°C or +4°C (n = 5 fish per treatment) for 28 d in flow-through seawater tanks located in the Crary Science and Engineering Center at McMurdo Station Antarctica.
Recombinant glycosyltransferases can be expressed in various expression systems.
We found that all 13 of the Populus NP-like genes belonging to either Group I or II are expressed in various tissues in both LD and SD conditions.
However, in the adult kidney and several other organs, Cox-2 is constitutively expressed in various cell types, and its expression increases under non-physiological conditions [ 4].
Under physiological conditions, MCP-1 is constitutively expressed in various types of cells, such as neurons, astrocytes, microglia, and endothelial cells at a minimal level.
S1PRs are differentially expressed in various tissues, and the individual S1PR expression level varies under different physiological and pathological conditions.
G250-antigen is also expressed in various other tumor types (e.g. cervix, lung) under hypoxic conditions.
