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expressed in the milk of transgenic goats has become available.
Harvesting natural silk is one option, but the genes that encode the protein can be transferred to other species, so that it can be produced by bacteria in fermentation vats, or even expressed in the milk of transgenic goats.
As β-lg is not expressed in the milk of all species, it is unlikely that its ability to bind FA is its primary function.
Given the fact that the recombinant human BChE expressed in the milk of transgenic goats is mainly in the dimer form [ 14], it is not surprised that the major eluted fusion protein peaks from the milk of transgenic mice and goats by SEC-HPLC are dimers in size.
The results indicated that the amount of the nerve agents required to inhibit 100% of enzyme activity (Fig. 3A) was in good agreement with that of plasma huBChE [ 4], as well as recombinant huBChE expressed in the milk of transgenic mice [ 14], suggesting that the fusion protein is similar to the plasma huBChE with comparable broad spectrum properties against a variety of nerve agents.
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In conclusion, we report here, for the first time, the co-microinjection with a 150-kb hLF BAC and a marker gene into donor cells followed by somatic cloning is very efficient in producing cattle mammary bioreactor, and rhLF was expressed in the milk reaching a level of 3.4 g/l.
Results showed that the majority of the genes involved in oligosaccharide metabolism are expressed in the milk somatic cells.
The ninety-two gexpressedessed in the milk samples had RPKM values ranging from 0.3 to 172.2.
Transcripts corresponding to immune cell markers were most abundantly expressed in the milk cell fraction, were moderately expressed in mammary tissue, and were minimally present in milk fat.
FUT5 was only expressed in the transition milk samples of Holstein animals.
A variety of recombinant human proteins, including immunoglobin, growth hormone and clotting factors, have been expressed and secreted in the milk of transgenic animals [ 39].
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