We now investigate the relationship between cells expressing these markers and use gene expression microarray analysis to identify genes differentially expressed in the cell populations.
Fgf10 was represented on the arrays and was expressed in the cell lines as expected from the expression patterns from in situ hybridisation at the equivalent stage of development [18].
HE4 was mainly expressed in the cell membrane, and the cytoplasm also showed slight expression.
The scFv fusion protein was found to be expressed in the cell lysate, culture supernatant, periplasmic extract, with highest expression in the inclusion bodies.
ZO-1 was found to be expressed in the cell sheets preserved in HBSS; however, staining for ZO-1 was weak for hOEC sheets preserved in PBS, and was entirely absent in those preserved in Optisol GS™ (Fig. 3B).
Until now, the microbial rhodopsins have only been expressed in the cell membrane, but Anselm Levskaya of the University of California, San Francisco, described how he is developing ways of using optogenetics to interrogate the computational processes that take place within cells.
Flow cytometric analysis of Gr-1 and F4/80 markers expressed in the cell surface of MDSCs.
Moreover, we can reconstruct these structures without any assumptions about the intermediate signalling network expressed in the cell or assumptions about signalling transfer mechanisms.
Once the NPΔ451 739 protein was expressed in the cell, it tends to oligomerize and gather adjacent molecules to form ring-like particles (Fig. 3_II), which would serve as the building block for further NLC assembly.
ErbB receptors are mainly expressed in the cell membrane.
Clearly, none of these kinases were phosphorylated and expressed in the cell lines tested.
