An additional consideration is that genes featuring poised polymerase at the promoter in many or most nuclei, but are only expressed in a few nuclei, will have weak signals at the body of the gene.
Consistent with results from in vitro cultured cells, CXXC4 was downregulated in tissues with high level of EZH2 expression, whereas it was highly expressed in tissues with weak EZH2 expression.
Most of the genes analysed, however, were either not detectable (no signal) or only weakly expressed in NCCIT cells (weak signals) and did not reveal any down- or upregulation after drug treatment.
Under the assumption that candidate genes for Pl ARG are constitutively expressed at a weak level in non-inoculated plants [ 14, 15], seedlings were grown in the greenhouse at a 14 h light (18°C /10 h dark (16°C) cycle for eight days.
Zebrafish nme7 is predominantly expressed in gonads and only a weak expression can be found in other studied tissue (Fig. 10).
Hence, the stronger signals obtained with Abcam2828 on immunostaining brain sections as well as on Western blots with brain extracts could either be due to a better reactivity of this antibody on MeCP2_e2 when it is expressed in vivo, or to a weak cross reaction on MeCP2_e1.
TGFβ was not detectable in the kidneys of wild-type mice, whereas in ITGA2 knockouts, TGFβ was primarily expressed in tubular cells, with a weak glomerular signal.
In contrast to CD133, CD44 was expressed to a weaker extent in human cancer cells in xenograft tissue and colospheres than in spheroids.
RAR-α, RAR-γ and RXR-α were expressed in all cells but weak expression of RAR-β could be detected in T3M-4 cells only.
In contrast, CPK2 was predominantly expressed in pollen, and showed weaker expression in roots, shoots, flowers, and siliques (fig. 7 B).
