Exact(1)
The original list of genes differently expressed generated by DESEQ was manually curated and only those contigs with >15 DESEQ-counts and over 2log2 fold-change were used.
Similar(7)
Expressed sequence tags (ESTs) are segments of expressed genes generated by single-pass sequencing of cDNA libraries [ 7].
Data of differentially expressed lncRNAs, generated by the microarray, has been deposited in Gene Expression Omnibus with an accession number GSE 50879 (http://www.ncbi.nlm.nih.gov/geo/info/linking.html).html
This is sensible in consideration of the fact that the number of differentially expressed genes generated by a given contrast depends on the sample sizes used in the experimental treatments being compared.
Lists of genes differentially expressed were generated by requiring that the absolute value of the fold change be higher than two, that the mean number of reads for each gene be higher than 145 for at least one of the genotype, and the adjusted P-value be lower than 0.01.
Lists of genes differentially expressed were generated by requiring that the absolute value of the fold change be higher than two, that the log2 of the expression level (or signal) for each gene be higher than six, and the adjusted P-value, using the Benjamini-Hochberg method, be lower than 0.005.
As the HASPB export pathway is conserved in higher eukaryotes, mammalian cell mutants expressing HASPB18 GFP (generated by random retroviral insertion mutagenesis) have been used to investigate the trafficking pathway (Stegmayer et al., 2005).
The final list of positive, phenotype-specific, differentially expressed genes was generated by removing all the genes that were also differentially expressed in the negative control population during the same transition.
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