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The plasmid is based on the pET expression vector system and induction of the T7 promoter leads to the expression of a highly expressed fusion protein (89 aa) followed by a higher affinity poly(His) region (53 aa) for purification and the C-terminal mimotope.
The main advantage of transcriptome sequencing over WGS is that it provides: gene expression level information, identification of expressed fusion transcripts, post-transcriptional modification including alternative splicing, single nucleotide variants (SNV) and RNA editing events [ 20].
The slowdown of folding, rather than the fast expression of fusion protein, should be a primary factor to drive the expressed fusion protein to inclusion bodies.
The expressed fusion proteins existed as inclusion bodies in the cytoplasm and the expression levels of the six AMPs reached up to 40% of the total cell protein content.
The results indicated that the expressed fusion protein existed as an inclusion body in the cytoplasm and the expression amounts of six peptide antibiotic fusions are all higher than 34% of the total cell protein.
The expressed fusion protein was smaller than the expected size of EGFP-NS3, 55 kDa.
The expressed fusion protein (IgG-FMDV) was purified and emulsified with oil adjuvant.
A recombinantly expressed fusion protein [(r OfC1Inh] was employed in demonstrating the anti-protease function of OfC1Inh.
The cloning of genes from marine organisms that encode fluorescent proteins provides a way to tag and monitor the intracellular behavior of expressed fusion proteins.
To enable electrochemiluminescence detection, the target proteins were prepared as bacterially expressed fusion proteins and captured on the surface of specially designed microtiter plates having integrated electrodes.
p65K-EGFP expressed fusion protein in a diffuse cytoplasmic pattern, and p85A-EGFP and p85B-EGFP produced a speckled pattern.
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