This fitness loss can be caused by particular functions carried on the plasmid which are detrimental upon expression in the new host (e.g. regulatory proteins interfering with the global gene expression network), or by proteins expressed from plasmid which have a direct phenotypic effect (e.g. antibiotic resistance) (Nguyen et al., 1989).
It signals, in fact, that an artist is getting to the core of what it means to be an artist – as in his often-quoted remarks about "the expression that there is nothing to express, nothing with which to express, nothing from which to express, no power to express, no desire to express, together with the obligation to express". Bram van Velde could have been made for Beckett.
In the "Three Dialogues" he wrote with Duthuit in 1949, he claimed to favor "the expression that there is nothing to express, nothing with which to express, nothing from which to express, no power to express, no desire to express, together with the obligation to express".
This accounts, in my case, for the odd tension between writing and laziness which Samuel Beckett describes to a T: "There is nothing to express, nothing with which to express, nothing from which to express, no power to express, no desire to express, together with the obligation to express".
And now and then the books do illustrate Beckett's dictum that "there is nothing to express, nothing with which to express, nothing from which to express, no power to express, no desire to express, together with the obligation to express".
The host protein integration host factor (IHF) was expressed from pRC188, which identical to pPR204 obtained from Phoebe Rice.
The U2/target RNA was expressed from pRS314/U2δStem/L2, which was generated by inserting a synthetic 36 bp dsDNA oligonucleotide (5'-CTAGAAGCTAATGTTTTGGCCTCTTCAAGATTATGG-3') into the NheI site of pRS314/U2δStem.
Wild type Tn10 transposase was expressed from pRC60 which contained the transposase gene on an NdeI-BamHI fragment cloned into pET11a.
Furthermore, the elongation was less distinct if eGFP was expressed from pJOE4056.2, which also achieved lower fluorescence compared to pWA21 (Fig. 2).
First, Xist does not spread efficiently when expressed from autosomes, which are relatively LINE-poor, and spreading is especially inhibited into LINE-poor autosomal areas (Popova et al. 2006; Tang et al. 2010).
