Sentence examples for expressed from each from inspiring English sources
As a result of these limitations, we set out to create a set of E. coli expression vectors that incorporate these essential features: i) protein expressed from each vector is initially purified in the same manner; ii) the cloning procedure is the same for all vectors and iii) a range of solubility tags are available.
Water expressed from each cut stem was collected using an Eppendorf tube containing dry cotton wool.
Elaborate regulation mechanisms result in numerous cDNA and protein isoforms being expressed from each gene of the p53 family.
The ligated products were transformed into E.coli HB2151 and the soluble scFv protein was expressed from each clone[39].
Approximately 30 kDa scFv protein was expressed from each of the three selected clones in the periplasmic extract sample compared to negative control (Fig. 4).
There also seems to be an upper limit of about 25 IS6110 elements per genome, possibly regulated by a trans acting repressor expressed from each element that limits the transpositional activity more the more elements are present [24].
We therefore investigated the RPS19 levels expressed from constructs with each of the two insertions (c.-147_-146insGCCA, c.-147_-146insAGCC) or the deletion (c.-144_-141delTTTC), respectively.
The events are treated as independent strata in the model, and time is expressed from randomization to each event.
Because the expression of many sRNAs is reported to be regulated under specific conditions [ 2, 3], such as under various stresses, in different kinds of media or in the presence of certain chemicals, we analysed the expression of each sRNA expressed from a prophage region using total RNAs prepared from cells grown under these conditions.
K562 cells were nucleofected with both WAS-specific ZFNs, each expressed from a separate plasmid, and a third plasmid encoding the donor template DNA (Fig. 1A).
The five major spliceosomal snRNAs are each expressed from multiple genes and (with the exception of U6) have multiple nucleotide differences.
