Your English writing platform
Discover LudwigSuggestions(1)
Exact(51)
Using an HSV-based amplicon vector system for transgene delivery, we demonstrate that: 1) ETR is a sensitive MR marker gene; 2) several transgenes can be efficiently expressed from a single amplicon; 3) expression of each transgene results in functional gene product; and 4) ETR gene expression correlates with expression of therapeutic genes when the latter are contained within the same amplicon.
As an immediate practical application we report the expression of a complete functional monoclonal antibody expressed from a single polypeptide, which was cleaved by our TEVp mutant into the two antibody chains and secreted as an assembled and functional molecule.
The mannitol biosynthetic genes were expressed from a single synthetic operon inserted into the cyanobacterial chromosome by homologous recombination.
However, due to our incomplete understanding of microRNA biogenesis, such "shRNAmirs" often fail to trigger potent knockdown, especially when expressed from a single genomic copy.
In this study, the 3 genes involved in the PHB pathway were expressed from a single vector in order to avoid the heterogeneity of plasmid distribution.
The two genes connected by IRES sequences can be expressed from a single promoter in IRES method used in this study.
Similar(9)
We were able to easily detect tagging of a number of endogenous proteins in both S. coelicolor and S. lividans when tmRNADD was expressed from a single-copy integrated gene or from a multicopy plasmid-borne gene.
Dicer was expressed from a single-copy plasmid.
ΔBAR represents worm FCHO-1 (amino acids 288 968) lacking the F-BAR domain expressed from a single-copy transgene while APA represents amino acids 454 565 overexpressed by an extrachromosomal (See Figure 6 ).
The dual-expression system is comprised of codon-optimized Cullin3 and Rbx1 genes co-expressed from a single pET-Duet-1 plasmid.
The expression of clustered miRNA is highly correlated, as they are often co-expressed from a single promoter as a single polycistronic transcript with neighbouring miRNA [ 10, 11].
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com