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This contrasts to previous studies in which the constitutive hacA was expressed from a highly-expressed promoter [ 43] or expressed from the pyrG locus [ 30].
MiR-19 repression of the 19RE reporter expression was defined as the luciferase activity expressed from the REm reporter divided by that expressed from the 19RE reporter.
Notably, BLIMP1 (expressed from 6.5-dpc mouse PGCs) was concomitantly upregulated, whereas STELLA expression (expressed from 7.5-dpc mouse PGCs) was not significantly altered.
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It was a message that echoed the skepticism expressed from London to Berlin and Tokyo to Seoul.
Both enzymes are recombinantly expressed from Azotobacter vinelandii.
Fold repression was calculated as normalized luciferase activity expressed from pREm-fLuc divided by that expressed from p19RE-fLuc.
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These observations could imply that PhaZ2, expressed from pER2, is more efficient in mobilizing PHB from granules than is PhaZ1, expressed from pER1.
In previous studies, recombinant telethonin expressed from E. coli was refolded in the presence of Z1Z2.
Imprinted genes are differentially expressed from the maternally and paternally inherited alleles.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com