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At the same time, there has been strong disapproval expressed for two senators who voted the plan down, Kelly Ayotte of New Hampshire and Jeff Flake of Arizona.
We stably expressed for two weeks a control aptamer (C6) or RG27.
Lin28 protein was detected as early as six hours after the first transfection, remained highly expressed for two to three days, and was detected up to five days after each transfection.
Odds ratios for a Kellgren-Lawrence score of 2 or more are expressed for two models: model 1 treats the twins as individuals; model 2 includes both the individual observations (OR shown) and the twin pair mean of the dietary variable (OR not shown).
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For pulse chases, Swe1-3myc was expressed for one hour following release from G1 arrest and glucose added to halt transcription, or was expressed for 1 hour in cdk1as1 cells arrested in G2 with 0.5 µM 1NM-PP1 after which cells were released back into the cell-cycle.
BJ13 is a clone in which telomerase flanked by loxP sites was expressed for seven doublings (beginning at population doubling (PD) 85, approximately five doublings before the parental culture senesced).
Both the 5′ and 3′ mature miRNAs were differentially expressed for eight miRNA precursors (miR-10b, miR-129, miR-1298, miR-142, miR-144, miR-148a, miR-302a, and miR-486).
For our variant analysis we chose to express for four hours at 30°C.
Cells were induced using 1 mL of 1M isopropyl b-D-1 thiogalactopyranoside and allowed to express for three hours.
Cells were transfected with pAct-FasIII using Effectene (Qiagen) and allowed to express for four days before fixation.
But the admiration expressed for the two former occupants of the White House also testifies to the fluidity of presidential reputations.
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