The use of Venn diagram assisted us in selecting the genes that were detected as differentially expressed for the comparison A (0.5% sucrose +1% starch versus 1% sucrose) and B (0.5% sucrose +1% starch versus 0.5% sucrose), excluding those genes that were detected in the comparison C (0.5% sucrose versus 1% sucrose) (which are not related to starch+sucrose effects).
We performed ANOVAs to identify genes that were differentially expressed for phenotypic comparisons of interest.
Transcripts differentially expressed for pairwise comparisons of Gh fiber at 10, 15, 18, 21, and 28 DPA.
Transcripts differentially expressed for pairwise comparisons of Gb fiber at 10, 15, 18, 21, and 28 DPA.
The effect of deterioration is expressed, for ease in comparison, to an increase in the required thickness of the jacket with expected service-life.
We tabulated the numbers of transcripts differentially expressed for each pairwise comparison and the numbers of upregulated transcripts in the developmental stages that comprise each pair (Table 1).
A gene was considered differentially expressed for a pairwise comparison if its adjusted p-value was less than or equal to 0.01.
Fig. 3 Venn diagram showing the number and relationship of proteins differentially expressed for the Substrate comparisons.
Additionally, DPAP1 is an ideal enzyme to demonstrate the value of our approach because we already identified a suitable ABP for this target [12], [13], we had specific information about its substrate specificity [10], [12], [14], and we had access to recombinantly expressed enzyme for comparison of our method with standard screening assays [14].
Beta-actin transcript levels are exemplarily shown for a highly expressed gene for comparison.
