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It remains unclear whether these genes were not expressed, expressed at different life stages, or expressed at levels below the detection threshold of the two techniques.
This meant that they were either not expressed, expressed at extremely low levels, or that the gene predictions were not correct and these genes were simply not there.
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Genes that originate from lineage-specific duplications (defined as inparalogs by Koonin [ 46]) exhibit discordant patterns of expression: most inparalogs are either not expressed or expressed at different levels.
Our results are consistent with human NME7 expression which is predominantly expressed in testis and expressed at significant levels in ovary and brain [ 15].
These results demonstrate the 4x tandem array of the SCBV-IM enhancer can increase transcript abundance in a stable transformed maize plant and in some cases may cause ectopic expression of genes that are not expressed, or expressed at very low levels.
While our results indicate that CD44 is highly expressed in OSE and expressed at significantly lower levels in CEPI, KIT expression was found to be low in all of our samples and thus not differentially expressed.
ASL3 showed tissue-specific expression, as it was highly expressed in the chlorenchyma, but expressed at much lower levels in roots and panicles.
Indeed, vimentin is normally not expressed or expressed at very low levels in mature neurons, but its expression is induced during axonal regeneration after injury [54].
PDGF receptors are not expressed, or expressed at low levels, in normal lung epithelial cells, however, expression of PDGFRα has been reported in lung cancer cell lines and tumor tissue [ 207- 209].
From these data it is evident that expression levels vary significantly, with Ngb being poorly expressed and Mb/SynHb being expressed at the highest concentrations.
Three target genes were weakly expressed or not expressed at all stages in both Emian22 and 3 79 (defined as no expression differences in this study).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com