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First, to test effects on the y2 phenotype, the weak but ubiquitously expressed driver Arm-Gal4 [35] was used to knockdown Top2 expression.
We next characterized the phenotypes of TG-RNAi flies using ubiquitously expressed driver (Da-GAL4>UAS-TG IR).
The following lines were used: Canton-S, Oregon-R, w (wild-type); the double-balancer If/Cyo; Sb/TM3, Ser; the ubiquitously expressed driver da-GAL4 (Bloomington Stock Center, Bloomington, IN); UAS-CREG-RNAiKK (line: 108999), UAS-cathepsin L-RNAiKK (110619), UAS-cathepsin B-RNAiKK (108315), VDRC (Vienna, Austria); Lsp2-GAL4-UAS-GFP-Atg8 [23].
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The exterior can express driver's opinions, endorse products or blend with surroundings.
Many of these studies focus on creating analytical models expressing driver behavior in relation to the characteristics of the roads [1, 2, 3, 4].
We demonstrated the efficacy of this method by identifying landing sites that are optimized to express driver transgenes in the adult nervous system.
Importantly, however, KD of the atg5/atg9 core autophagy components using these MB expressing drivers significantly impaired STM scores (Fig. 2e g).
UAS-RNAi stocks balanced with CyO GFP w+ were crossed to a variety of Gal4 expressing drivers: Act5C-Gal4 (Y. Hiromi, Bloomington Stock centre); Da-Gal4 and 24B-Gal4 (gifromfroM.M. Bienz); Elav-Gal4; Mef2-Gal4 (a gift from S. Huelsman).
We first used the ubiquitously expressed daGAL4 driver to drive expression of peroxisomal Catalase (UAS-Cat +).
In this screen we utilized the Smn73Ao allele in cis with a ubiquitously expressed tubulinGAL4 driver (Lee and Luo, 1999).
Fertility was rescued in vret mutant females and males by a single copy of vret using a UASp-vret-myc transgene driven by the ubiquitously expressed actin5C-Gal4 driver (see Table S3 in the supplementary material).
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