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These three scFv were also expressed, displayed on phage after infection of E. coli TG1, and the immunoreactivity of each phage solution controlled by ELISA on trastuzumab F ab′ 2 fragments (data not shown).
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Cell surface display technologies are also in vivo display technologies and use bacteria [177, 178], yeast [179, 180] and mammalian cells [181] as host cells, in which the fusion gene resulting from a protein gene and a partial (or full) endogenous cell surface protein gene is expressed and displayed on the cell surface.
Both analysis provided evidence that VP6 was efficiently expressed and displayed on the cell surface of L. lactis.
We successfully developed a Pichia pastoris surface display system utilizing flocculation functional domain of FLO protein, and had the lipase LipB52 expressed and displayed on Pichia pastoris KM71 cell surface.
A Pichia pastoris surface display system utilizing flocculation functional domain of FLO protein was developed, and the activity lipase LipB52 was expressed and displayed on Pichia pastoris KM71 cell surface.
A GFP fusion with the LysM-containing endo-β N-acetylglucosaminidase (Mbg) from B. thuringiensis was successfully expressed and displayed on the whole cell surface of B. thuringiensis cells (Shao et al. 2012).
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The results obtained so far with L. lactis NZ9000/pCWA:VP6 indicate that the VP6 protein is efficiently expressed and correctly displayed on the cell wall and that specific antibodies can be elicited, demonstrating correct folding of the epitopes and good immunogenicity.
In this background, ectopically expressed Vsp proteins are prominently displayed on the surface without much interference from other proteins, therefore leading to a pronounced change in phenotype.
The antibody genes are expressed and the gene products displayed on the surface of filamentous bacteriophage as fusion proteins [ 7, 11, 22- 25].
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